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TracePure™ Plasmid Miniprep Kit
For purification of plasmid DNA from a lab scale Escherichia coli cultivation
REF: TP0222
- Rapid : 30 min
- High purification : A260/280 ~1.8. Uncontaminated with gDNA and RNA.
- High recovery : up to 25 µg
- Stability : 12 months (Room temperature)
- Shipping conditions: Ambient temperature
- ISO 13485
Description
TracePureTM Plasmid Miniprep Kit provides a rapid and affordable method for plasmid DNA purification from small scale Escherichia coli cultivation. With the utilization of silica-based technology compatible in a Spin Column, up to 25 µg of pure plasmid DNA can be recovered.
Purified plasmid DNA can be used directly in enzymatic digestion, PCR, qPCR, cloning, electrophoresis, transformation and sequencing without further manipulation.
Components
| Buffer S1 |
| Buffer L2 |
| Buffer N3 |
| Wash Buffer 2 (concentrates) |
| Elution Buffer |
| RNAse A (10 mg/mL) |
| Spin Columns preassembled with 2 mL Collection Tubes |
| Collection Tube 1.5 mL |
Notes
- In case of using EndA+ strains, an additional Wash Buffer 1 (not include in the kit) is required. Order seperately reagent Wash Buffer 1 with REF: RGWB003. Aside from this case, skip the step using Wash 1.
- Ensure ethanol is added to Wash Buffer 2.
- Add RNase A (0.15 mL) to 15 mL Buffer S1 before use, preserve the reagent at 2 – 8°C.
Thông số kỹ thuật
| Technology | Silica-based technology compatible in a Spin Column |
| Method | Centrifuge |
| Pack Size | 50 preps, 100 preps , 150 preps |
| Sampe volume for high-copy plasmid | 1,5 – 5 mL |
| Sampe volume for low-copy plasmid | 1,5 – 10 mL |
| Elution volume | 30 – 100 µL |
| Target | Plasmid DNA |
| Applications | PCR, Cloning, Sequencing, enzymatic digestion, Nucleic acid labelling… |
| Storage | RNAse A: 2 – 8oC. Other components: Room temperature |
| Expiry date | 12 months |
