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TraceBead™ Bacterial gDNA Extraction Kit
For purification of genomic DNA from bacterial inoculation, colonies, clinical samples (eg. tuberculosis) including plasma, serum, cell-free body fluids (urine, oral and nasal fluids, and urogenital fluid) and whole blood.
REF: TB0222
- Semi-automation/automation
- High purification: A260/280 ~ 1.8.
- High recovery: up to 100 µg
- Stability: 12 months (Room temperature).
Proteinase K: 2 – 8o Lyzozyme: – 20oC - Shipping conditions: Ambient temperature
Riêng Proteinase K, Lyzozyme: – 20oC - ISO 13485
| Technology | Silicon dioxide-coated magnetic bead |
| Process time | 30 – 45 min, depends on automated extraction instruments |
| Sample volume | 50 – 300 µL |
| Elution volume | 100 µL |
| Target | Bacterial genomic DNA |
| Applications | PCR/RT-PCR, real-time PCR/real-time RT-PCR |
| Storage | Proteinase K: 2 – 8oC. Lyzozyme: – 20oC. Other components: Room temperature |
| Expiry date | 12 months |
TraceBead™ Bacterial gDNA Extraction Kit employs magnetic bead technology for a rapid and efficient extraction of genomic DNA with high purification and recovery from various sample types including whole blood, plasma, serum, oral and nasal fluids, urine, urogenital fluid, bacterial inoculation, colonies, clinical samples (consist of tuberculosis-infected samples).
Nucleic acids after being isolated by TraceBead™ Bacterial gDNA Extraction Kit can be used directly in PCR, real-time PCR and other downstream applications.
Notes
- Add 80 mL ethanol 96 – 100% into Wash Buffer 3 bottle before use.
- Several sample types need pre – lysis preparation before being transferred to 96 well plate for main extraction process: See details in USER GUIDE.
Components
| Lysis Buffer |
| Wash Buffer 1 |
| Wash Buffer 2 |
| Wash Buffer 3 (concentrated) |
| Bead Suspension Buffer |
| Elution Buffer |
| Proteinase K |
| Lysozyme |
| Magnetic Beads |
| Tip comb |
| Deep well 96 |
Quick Guide_ TraceBeadTM Bacterial gDNA_ENG
Flyer_ TraceBeadTM Bacterial gDNA _ENG
